1ICM

ESCHERICHIA COLI-DERIVED RAT INTESTINAL FATTY ACID BINDING PROTEIN WITH BOUND MYRISTATE AT 1.5 A RESOLUTION AND I-FABPARG106-->GLN WITH BOUND OLEATE AT 1.74 A RESOLUTION

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	36.050, 51.800, 31.480
Unit cell angles	90.00, 92.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.500
R-factor	0.159
RMSD bond length	0.010
RMSD bond angle	2.690
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.500 *
Rmerge	0.047 *
Total number of observations	34869 *
Number of reflections	12574 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1 *	17 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.7 (mM)
2	1	drop	PIPES	100 (mM)
3	1	drop	PEG4000	34-37 (%)
4	1	reservoir	PEG4000	34-37 (%)