1ICE
STRUCTURE AND MECHANISM OF INTERLEUKIN-1BETA CONVERTING ENZYME
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 64.400, 64.400, 163.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.600 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.820 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 * |
| High resolution limit [Å] | 2.600 * |
| Rmerge | 0.083 * |
| Completeness [%] | 90.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | citrate | 50 (mM) | |
| 3 | 1 | drop | DTT | 2.0 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 15 (%) | |
| 5 | 1 | reservoir | 400 (mM) | ||
| 6 | 1 | reservoir | sodium HEPES | 200 (mM) | |
| 7 | 1 | reservoir | sodium cacodylate | 5 (mM) | |
| 8 | 1 | reservoir | beta-octyl glucoside | 0.5 (%) |
