1IC7

CRYSTAL STRUCTURE OF HYHEL-10 FV MUTANT(HD32A99A)-HEN LYSOZYME COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1999-07-01
Detector	MACSCIENCE
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	57.215, 57.215, 237.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
Rwork	0.177
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	0.038
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.058	0.342
Total number of observations	153223 *
Number of reflections	23833
<I/σ(I)>	11.2
Completeness [%]	98.9	98.9
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.6	293	HEPES, PEG6000, MPD, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	HEPES	100 (mM)
2	1	1	PEG6000	9-11 (%(w/v))
3	1	1	MPD	7-9 (%(w/v))