1IC2
DECIPHERING THE DESIGN OF THE TROPOMYOSIN MOLECULE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.908 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.400, 45.500, 56.300 |
| Unit cell angles | 93.70, 98.10, 104.40 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.249 * |
| Rwork | 0.249 |
| R-free | 0.28350 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 13.170 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MLPHARE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.139 |
| Number of reflections | 26797 | |
| <I/σ(I)> | 15.4 | 10 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 10 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 | 277 | used macroseeding * |
| 1 | VAPOR DIFFUSION | 7.6 | 277 | used macroseeding * |
| 1 | VAPOR DIFFUSION | 7.6 | 277 | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG200 | 17 (%) | |
| 2 | 1 | reservoir | isopropanol | 10 (%) | |
| 3 | 1 | reservoir | 16 (mM) | ||
| 4 | 1 | reservoir | ammonium acetate | 70 (mM) | |
| 5 | 1 | reservoir | Tris |
