1IBJ
Crystal structure of cystathionine beta-lyase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.83 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 107.750, 154.332, 118.763 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.2495 |
| Rwork | 0.249 |
| R-free | 0.30900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qgn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.870 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.135 |
| Total number of observations | 102185 * | |
| Number of reflections | 37727 * | |
| <I/σ(I)> | 22.3 | 3.1 |
| Completeness [%] | 94.3 | 81.6 |
| Redundancy | 3.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | Ammonium sulfate, Mes-NaOH, PLP, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | |
| 3 | 1 | drop | PLP | 0.01 (mM) | |
| 4 | 1 | drop | reservoir solution | 2 micro litter | |
| 5 | 1 | reservoir | MES/NaOH | 100 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 300 (mM) |
