1IBG

STRUCTURE AND SPECIFICITY OF THE ANTI-DIGOXIN ANTIBODY 40-50

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	93.660, 84.770, 70.080
Unit cell angles	90.00, 127.95, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.209
Rwork	0.209
RMSD bond length	0.015
RMSD bond angle	28.500 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.430 *
Rmerge	0.049 *
Total number of observations	24232 *
Number of reflections	12688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop		1 (mM)
3	1	drop	PEG3350	15 (%(w/v))
4	1	drop	ouabain	4.5 (mM)
5	1	drop	Tris-HCl	50 (mM)
6	1	reservoir	PEG3350	15 (%(w/v))
7	1	reservoir	Tris-HCl	50 (mM)