1IAR

INTERLEUKIN-4 / RECEPTOR ALPHA CHAIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	49.720, 67.980, 108.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.300
Rwork	0.218
R-free	0.28900
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	25.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	MLPHARE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.038 *	0.067 *
Number of reflections	16897
<I/σ(I)>	52	20
Completeness [%]	99.7	99.2
Redundancy	5.8	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	sodium acetate	25 (mM)
3	1	drop		120 (mM)
4	1	reservoir	ethanol	20 (%(v/v))
5	1	reservoir	methylpentanediol	5 (%(v/v))