1IAP
CRYSTAL STRUCTURE OF P115RHOGEF RGRGS DOMAIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-01-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9760 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.859, 37.432, 128.171 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.550 - 1.900 |
R-factor | 0.219 |
Rwork | 0.219 |
R-free | 0.27500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | SE-MET STRUCTURE OF THE SAME PROTEIN SOLVED BY MAD PHASING FROM A FOUR-WAVELENGTH MAD DATASET COLLECTED AT CHESS F2 BEAMLINE (UNPUBLISHED DATA) |
RMSD bond length | 0.005 |
RMSD bond angle | 1.000 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.120 | 0.485 |
Number of reflections | 13303 | |
<I/σ(I)> | 12.1 | 4.8 |
Completeness [%] | 90.0 | 79.6 |
Redundancy | 3.8 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 9.5 | 20 * | PEG 3350, ammonium formate, CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG3350 | 18-22 (%(w/v)) | |
2 | 1 | reservoir | CHES | 83 (mM) | |
3 | 1 | reservoir | ammonium formate | 80-240 (mM) |