1IAO

CLASS II MHC I-AD IN COMPLEX WITH OVALBUMIN PEPTIDE 323-339

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1997-04
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	101.300, 101.300, 92.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.000 - 2.600
R-factor	0.255
Rwork	0.255
R-free	0.32000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dlh
RMSD bond length	0.012
RMSD bond angle	29.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.057	0.383
Number of reflections	14777	1690 *
<I/σ(I)>	10	2
Completeness [%]	99.0	91
Redundancy	9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.5	22 *	32% PEG 600, 0.1 IMIDAZOLE MALATE, PH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-9 (mg/ml)
2	1	reservoir	PEG600	32 (%)
3	1	reservoir	imidazole malate	0.1 (M)