1IAL

IMPORTIN ALPHA, MOUSE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1998-05
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	78.549, 89.675, 100.562
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.500
R-factor	0.189
Rwork	0.189
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bk5
RMSD bond length	0.005
RMSD bond angle	22.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.123	0.631
Total number of observations	144864 *
Number of reflections	24615 *
<I/σ(I)>	9.4	1.7
Completeness [%]	97.0	100
Redundancy	5.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		100 (mM)
4	1	drop	dithiothreitol	2 (mM)
5	1	reservoir	sodium citrate	0.8 (M)
6	1	reservoir	dithiothreitol	10 (mM)