1IAJ
CRYSTAL STRUCTURE OF THE ATYPICAL PROTEIN KINASE DOMAIN OF A TRP CA-CHANNEL, CHAK (APO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-05-30 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 108.270, 138.200, 113.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.29200 |
| Structure solution method | MIR |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.049 | 0.172 |
| Number of reflections | 20775 | |
| <I/σ(I)> | 22.4 | 3.7 |
| Completeness [%] | 97.4 | 94.2 |
| Redundancy | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | HEPES, PEG 4000, 2-propanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 100 (mM) | |
| 3 | 1 | drop | 2-propanol | 5 (%(v/v)) | |
| 4 | 1 | drop | PEG4000 | 5 (%(w/v)) | |
| 5 | 1 | reservoir | sodium HEPES | 100 (mM) | |
| 6 | 1 | reservoir | 2-propanol | 10 (%(v/v)) | |
| 7 | 1 | reservoir | PEG4000 | 5 (%(w/v)) |
