1IA9
CRYSTAL STRUCTURE OF THE ATYPICAL PROTEIN KINASE DOMAIN OF A TRP CA-CHANNEL, CHAK (AMPPNP COMPLEX)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2000-08-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.96487 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 109.270, 136.550, 113.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.24 * |
| Rwork | 0.240 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.185 |
| Number of reflections | 53642 | |
| <I/σ(I)> | 19.9 | 4.6 |
| Completeness [%] | 94.1 | 92.1 |
| Redundancy | 4.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 20 * | used macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | AMP-PNP-Mg2 | 1 (mM) | |
| 3 | 1 | drop | sodium HEPES | 100 (mM) | |
| 4 | 1 | drop | ammonium dihydrogen phosphate | 0.8 (M) | |
| 5 | 1 | reservoir | sodium HEPES | 100 (mM) | |
| 6 | 1 | reservoir | ammonium dihydrogen phosphate | 0.8 (M) |
