1I9Y
CRYSTAL STRUCTURE OF INOSITOL POLYPHOSPHATE 5-PHOSPHATASE DOMAIN (IPP5C) OF SPSYNAPTOJANIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2000-10-11 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.967, 67.407, 51.443 |
Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.195 * |
Rwork | 0.195 |
R-free | 0.22500 |
Structure solution method | SIRAS |
RMSD bond length | 0.018 * |
RMSD bond angle | 1.940 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.039 | 0.161 * |
Total number of observations | 117178 * | |
Number of reflections | 32930 * | |
<I/σ(I)> | 17 | |
Completeness [%] | 99.3 | 96.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 8 | 4 * | sodium chloride, tris, 2-mercaptoethanol, pH 8.0, MICRODIALYSIS, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 8 (mg/ml) | |
2 | 1 | 2 | 150 (mM) | ||
3 | 1 | 2 | Tris-HCl | 20 (mM) | |
4 | 1 | 2 | MPD | 10 (mM) |