1I9X
CRYSTAL STRUCTURE OF BPS-U2 SNRNA DUPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-10 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.750, 42.400, 33.140 |
| Unit cell angles | 90.00, 92.87, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.180 |
| R-factor | 0.253 * |
| Rwork | 0.253 |
| R-free | 0.27600 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.260 |
| High resolution limit [Å] | 2.000 | 2.180 |
| Rmerge | 0.048 | 0.384 |
| Total number of observations | 20240 * | |
| Number of reflections | 4125 | |
| <I/σ(I)> | 30.1 | 2.6 |
| Completeness [%] | 96.0 | 94 |
| Redundancy | 4.9 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 30 * | MPD, Spermine, Glycerol, tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Spermine | ||
| 3 | 1 | 1 | Glycerol | ||
| 4 | 1 | 1 | tris | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 2-5 (mg/ml) | |
| 2 | 1 | reservoir | MPD | 10 (%) | |
| 3 | 1 | reservoir | glycerol | 10-25 (%) | |
| 4 | 1 | reservoir | Tris | 25 (mM) | |
| 5 | 1 | reservoir | spermine | 1-3 (mM) |
