1I9G
CRYSTAL STRUCTURE OF AN ADOMET DEPENDENT METHYLTRANSFERASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.420, 86.790, 138.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.980 |
| R-factor | 0.205 * |
| Rwork | 0.205 |
| R-free | 0.23600 |
| Structure solution method | MIR |
| RMSD bond length | 0.100 |
| RMSD bond angle | 0.020 |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.980 |
| Rmerge | 0.049 * |
| Total number of observations | 70596 * |
| Number of reflections | 27574 |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 25 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | drop | AdoMet | 5 (mM) | |
| 5 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 6 | 1 | reservoir | lithium sulfate | 50 (mM) | |
| 7 | 1 | reservoir | PEG4000 | 16-18 (%(w/v)) |
