1I9C
GLUTAMATE MUTASE FROM CLOSTRIDIUM COCHLEARIUM: COMPLEX WITH ADENOSYLCOBALAMIN AND SUBSTRATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 103 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.348, 113.139, 108.439 |
| Unit cell angles | 90.00, 96.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.168 * |
| Rwork | 0.168 |
| R-free | 0.22100 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | 1ccw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.073 | 0.281 |
| Total number of observations | 555148 * | |
| Number of reflections | 121680 | 8102 * |
| <I/σ(I)> | 18.4 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 * | 293 | PEG-4000, cadmium chloride, coenzyme B12, sodium glutamate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | coenzyme B12 | 0.5 (mg/ml) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | reservoir | PEG4000 | 6 (%(w/v)) | |
| 5 | 1 | reservoir | Na-S-glutamate | 0.1 (M) | pH4.5 |
