1I8V
CRYSTAL STRUCTURE OF RNASE SA Y80F MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.044 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.030, 64.780, 78.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.600 - 1.250 |
| R-factor | 0.132 |
| Rwork | 0.130 |
| R-free | 0.15600 |
| Structure solution method | STARTING MODEL WAS USED WITHOUT MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rgg |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | STARTING (MODEL WAS USED WITHOUT MOLECULAR REPLACEMENT) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.650 | 1.260 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.057 | 0.426 |
| Number of reflections | 52680 | |
| <I/σ(I)> | 29.09 | 2.28 |
| Completeness [%] | 94.3 | 83.5 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | used microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | phosphate | 0.2 (M) | |
| 2 | 1 | drop | protein | 20 (mg/ml) | |
| 3 | 1 | reservoir | ammonium sulfate | 22-24 (%(v/v)sat) |
