1I8P
STRUCTURE DETERMINATION OF THE FERROCYTOCHROME C2 FROM RHODOPSEUDOMONAS PALUSTRIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.119, 71.706, 67.357 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.1942 |
| Rwork | 0.161 |
| R-free | 0.23700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fj0 |
| RMSD bond length | 0.029 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.175 | 0.599 |
| Total number of observations | 106868 * | |
| Number of reflections | 34702 | |
| <I/σ(I)> | 6.4 | 2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 293 | 42% AMMONIUM SULPHATE,0.1 M SODIUM CITRATE PH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
