1I8K
CRYSTAL STRUCTURE OF DSFV MR1 IN COMPLEX WITH THE PEPTIDE ANTIGEN OF THE MUTANT EPIDERMAL GROWTH FACTOR RECEPTOR, EGFRVIII, AT LIQUID NITROGEN TEMPERATURE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-06-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9789 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.500, 44.800, 108.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.800 |
R-factor | 0.175 |
Rwork | 0.172 |
R-free | 0.22500 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 2.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MERLOT |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 6.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.052 | 0.143 |
Total number of observations | 25654 * | |
Number of reflections | 24641 * | |
<I/σ(I)> | 18.6 | |
Completeness [%] | 98.5 * | 95.7 * |
Redundancy | 7.26 | 6.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 18 * | sodium chloride, MPD, PEG 10K, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | peptide | 4 (mg/ml) | |
2 | 1 | drop | ADA buffer | 40 (mM) | pH7.0 |
3 | 1 | drop | 25 (mM) | ||
4 | 1 | drop | MPD | 1 (%(w/v)) | |
5 | 1 | drop | PEG10000 | 6 (%(w/v)) | |
6 | 1 | reservoir | ADA buffer | 200 (mM) | pH7.0 |
7 | 1 | reservoir | MPD | 2-3 (%) | |
8 | 1 | reservoir | PEG4000 | 16 (%) |