1I8F
THE CRYSTAL STRUCTURE OF A HEPTAMERIC ARCHAEAL SM PROTEIN: IMPLICATIONS FOR THE EUKARYOTIC SNRNP CORE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2000-11-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.261, 95.738, 62.157 |
| Unit cell angles | 90.00, 92.69, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.235 * |
| Rwork | 0.235 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 26.232 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.040 | 0.723 |
| Number of reflections | 62547 | |
| <I/σ(I)> | 44.4 | |
| Completeness [%] | 98.5 | 97.2 |
| Redundancy | 6.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.3 | 293 | PEG-4000, acetate, glycerol, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
