1I7Z
ANTIBODY GNC92H2 BOUND TO LIGAND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-05-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.039 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 142.020, 64.070, 129.370 |
| Unit cell angles | 90.00, 123.06, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.219 * |
| Rwork | 0.220 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nsn |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.082 | 0.512 |
| Total number of observations | 164865 * | |
| Number of reflections | 43057 | |
| <I/σ(I)> | 16.9 | 2.4 |
| Completeness [%] | 98.9 | 85.7 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 4.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 25-30 (%(w/v)) | |
| 2 | 1 | 1 | 0.2 (M) | ||
| 3 | 1 | 1 | acetate | 0.1 (M) | pH4.5 |
