1I7Z

ANTIBODY GNC92H2 BOUND TO LIGAND

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-05-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.039
Spacegroup name	C 1 2 1
Unit cell lengths	142.020, 64.070, 129.370
Unit cell angles	90.00, 123.06, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.219 *
Rwork	0.220
R-free	0.26500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nsn
RMSD bond length	0.007 *
RMSD bond angle	1.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.082	0.512
Total number of observations	164865 *
Number of reflections	43057
<I/σ(I)>	16.9	2.4
Completeness [%]	98.9	85.7
Redundancy	3.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	25-30 (%(w/v))
2	1	1		0.2 (M)
3	1	1	acetate	0.1 (M)	pH4.5