1I7Y

CRYSTAL STRUCTURE OF C-PHYCOCYANIN OF SYNECHOCOCCUS VULCANUS AT 2.5 ANGSTROMS.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	2000-09-18
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	188.432, 188.432, 61.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 - 2.500
R-factor	0.202 *
Rwork	0.202
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1CBC SUBUNITS A AND B
RMSD bond length	0.007
RMSD bond angle	17.860 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.090	0.368
Total number of observations	112515 *
Number of reflections	14116
<I/σ(I)>	17.6	4.8
Completeness [%]	97.5	99.3
Redundancy	5.6	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	22 *	5% PEG4000, 50mM HEPES, 5mg/ml phycocyanin, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG4000	5 (%)
2	1	drop	Tris	50 (mM)
3	1	drop	protein	2.5-5 (mg/ml)