1I7W

BETA-CATENIN/PHOSPHORYLATED E-CADHERIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2000-06-26
Detector	ADSC QUANTUM 4
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	168.600, 85.300, 115.100
Unit cell angles	90.00, 107.90, 90.00

Refinement procedure

Resolution	100.000 - 2.000
R-factor	0.206 *
Rwork	0.206
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Armadillo repeat domain of murine beta-catenin PDB code 2bct
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.040	0.339
Total number of observations	287321 *
Number of reflections	101570
<I/σ(I)>	12
Completeness [%]	96.7	94.9
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	20 *	polyethylene glycol monomethylether 5000, Tris-HCl, NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-10 (mg/ml)
2	1	reservoir	PEG5000	13.5 (%(w/v))
3	1	reservoir	Tris-HCl	200 (mM)	pH8.0
4	1	reservoir		1.1 (M)