1I7R
CRYSTAL STRUCTURE OF CLASS I MHC A2 IN COMPLEX WITH PEPTIDE P1058
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-06-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0789 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.860, 63.820, 75.870 |
| Unit cell angles | 82.09, 76.20, 78.13 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.266 |
| Rwork | 0.264 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 2.169 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.084 | 0.232 |
| Number of reflections | 44322 | |
| <I/σ(I)> | 7.7 | 2.8 |
| Completeness [%] | 96.7 | 93.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG6000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
