1I7N
CRYSTAL STRUCTURE ANALYSIS OF THE C DOMAIN OF SYNAPSIN II FROM RAT BRAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 1999-11-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 120.412, 120.412, 165.456 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 11.980 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1auv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.940 |
| High resolution limit [Å] | 1.800 | 1.900 |
| Rmerge | 0.042 | 0.435 |
| Number of reflections | 61099 | |
| <I/σ(I)> | 40.9 | 3.1 |
| Completeness [%] | 97.4 | 94.6 |
| Redundancy | 10.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 294 | PEG 4000, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
