1I7L
CRYSTAL STRUCTURE ANALYSIS OF THE COMPLEX OF THE C DOMAIN OF SYNAPSIN II FROM RAT WITH ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2000-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.943 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 121.275, 121.275, 165.891 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 2.350 |
| Rwork | 0.220 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SYNCII model based on PDB ENTRY 1AUV |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.370 |
| High resolution limit [Å] | 2.260 | 2.310 |
| Rmerge | 0.083 | 0.474 |
| Number of reflections | 31094 | |
| <I/σ(I)> | 31.6 | 4.67 |
| Completeness [%] | 95.9 | 96.9 |
| Redundancy | 10.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 294 | PEG 4000, sodium chloride, TRIS, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
