1I7A
EVH1 DOMAIN FROM MURINE HOMER 2B/VESL 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.716, 50.014, 73.098 |
| Unit cell angles | 90.00, 101.19, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.240 |
| R-factor | 0.235 * |
| Rwork | 0.235 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DDW (SAME MOLECULE IN DIFFERENT PACKING DERIVED FROM HOMER 1B FROM RAT |
| RMSD bond length | 0.068 |
| RMSD bond angle | 1.186 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.280 |
| Number of reflections | 22446 | |
| <I/σ(I)> | 15.4 | 3 |
| Completeness [%] | 95.2 | 88.2 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 293 | 1 M SODIUM CITRATE, O.1 M CHES, pH 9.8, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 1.6 (M) | |
| 2 | 1 | 1 | HEPES | 0.1 (M) | |
| 3 | 1 | 1 | NAD | 10 (mM) |
