1I6N
1.8 A Crystal structure of IOLI protein with a binding zinc atom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-12 |
| Detector | SBC-2 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.015, 104.616, 48.462 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.800 |
| R-factor | 0.201 * |
| Rwork | 0.201 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i60 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.223 |
| Number of reflections | 35623 | |
| <I/σ(I)> | 22.5 | 6.1 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 6.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 * | 298 | Ammonium sulfate, Tris buffer, sodium chloride, EDTA, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | imidazole | 250 (mM) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.4 |
| 3 | 1 | drop | 0.15 (mM) | ||
| 4 | 1 | drop | 2.5 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.50-1.75 (M) | |
| 6 | 1 | reservoir | Tris | 0.050 (M) | pH8.00 |
| 7 | 1 | reservoir | 0.10 (M) | ||
| 8 | 1 | reservoir | EDTA | 1.0 (mM) | |
| 9 | 1 | reservoir | dithiothreitol | 3.0 (mM) |
