1I5P

INSECTICIDAL CRYSTAL PROTEIN CRY2AA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1993-05-22
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	85.600, 85.600, 163.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.970 - 2.200
R-factor	0.183
Rwork	0.183
R-free	0.23100
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.970	2.340
High resolution limit [Å]	2.200	2.200
Rmerge	0.057	0.131
Total number of observations	245580 *
Number of reflections	31509
<I/σ(I)>	13.7
Completeness [%]	99.2	98.9
Redundancy	8.0 *	7.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	298	Tris-HCL, EDTA, NH4OH, mercaptoethonanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	Tris-HCl	10 (mM)
3	1	reservoir	EDTA	1 (mM)