1I5P
INSECTICIDAL CRYSTAL PROTEIN CRY2AA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-05-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.600, 85.600, 163.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.970 - 2.200 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.23100 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.970 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.131 |
| Total number of observations | 245580 * | |
| Number of reflections | 31509 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 99.2 | 98.9 |
| Redundancy | 8.0 * | 7.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | Tris-HCL, EDTA, NH4OH, mercaptoethonanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | EDTA | 1 (mM) |
