1I5D
STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH TNP-ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 85.190, 85.190, 73.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.890 - 2.900 |
| R-factor | 0.25 * |
| Rwork | 0.263 |
| R-free | 0.30800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b3q |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.040 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.054 | 0.269 |
| Number of reflections | 7933 | |
| <I/σ(I)> | 30 | 8 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 25 * | sodium acetate 0.1 M Ammonium sulfate 1.9 M, pH 4.7. VAPOR DIFFUSION, HANGING DROP at 298 K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50-80 (mg/ml) | |
| 2 | 1 | drop | nucleotide | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.8-2.0 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
