1I4W
THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.418, 44.684, 99.819 |
| Unit cell angles | 90.00, 110.23, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.600 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.27400 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | ISIR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 * | 0.170 |
| Total number of observations | 202625 * | |
| Number of reflections | 10896 | |
| <I/σ(I)> | 19.5 | 4.6 |
| Completeness [%] | 90.8 | 63.4 |
| Redundancy | 5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | Schubot, G.S., (2000) Acta Crystallogr., D56, 902. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | 300 (mM) | ||
| 3 | 1 | reservoir | glycerol | 10 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 50 (mM) |
