1I3S
THE 2.7 ANGSTROM RESOLUTION CRYSTAL STRUCTURE OF A MUTATED BACULOVIRUS P35 AFTER CASPASE CLEAVAGE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 170 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 77.608, 89.303, 136.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.490 - 2.700 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i3p |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.070 | 0.334 |
| Total number of observations | 183500 * | |
| Number of reflections | 25271 | |
| <I/σ(I)> | 450.7 | |
| Completeness [%] | 94.9 | 96.3 |
| Redundancy | 7.26 | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 25 * | 9-13% PEG 20,000, 100-400 mM NaCl, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 100 (mM) | ||
| 10 | 1 | reservoir | MES | 100 (mM) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | HEPES | 20 (mM) | |
| 6 | 1 | drop | protein | 5-8 (mg/ml) | |
| 7 | 1 | drop | MPD | 1-8 (%) | |
| 8 | 1 | reservoir | 100-400 (mM) | ||
| 9 | 1 | reservoir | PEG20000 | 9-13 (%) |
