1I3A
RNASE HII FROM ARCHAEOGLOBUS FULGIDUS WITH COBALT HEXAMMINE CHLORIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 94 |
| Detector technology | CCD |
| Collection date | 2000-04-17 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.863 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 74.555, 74.555, 139.684 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.750 - 2.150 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ARNASE HII refined model (rcsb012848) |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 1.170 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.079 | 0.414 |
| Total number of observations | 82747 * | |
| Number of reflections | 13210 | |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 98.2 | 93.3 |
| Redundancy | 6.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | drop consists of equal amounts of protein and precipitant solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | reservoir | mPEG5000 | 10 (%) | precipitant |
| 3 | 1 | reservoir | butanol | 10 (%) | precipitant |
| 4 | 1 | reservoir | sodium citrate | 20 (mM) | precipitant |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | precipitant |
