1I31
MU2 ADAPTIN SUBUNIT (AP50) OF AP2 CLATHRIN ADAPTOR, COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM AT 2.5 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 64 |
| Unit cell lengths | 125.727, 125.727, 74.634 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| Rwork | 0.247 |
| R-free | 0.25470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bw8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.055 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.520 |
| Rmerge | 0.077 | 0.599 |
| Number of reflections | 21919 | |
| <I/σ(I)> | 25.8 | 1.6 |
| Completeness [%] | 96.4 | 76.2 |
| Redundancy | 50.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 291 | SODIUM FORMATE, SODIUM ACETATE, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
