1I2X

2.4 A STRUCTURE OF A-DUPLEX WITH BULGED ADENOSINE, SPERMIDINE FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-26
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	27.690, 47.710, 113.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.249 *
Rwork	0.249
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013 *
RMSD bond angle	2.030 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.400
Rmerge	0.079 *
Number of reflections	6180 *
Completeness [%]	94.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	298	spermidine, MPD. pH 6.0, VAPOR DIFFUSION, SITTING DROP at 298

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	spermidine
2	1	1	MPD
3	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA	0.72 (mM)
2	1	reservoir	sodium cacodylate	20 (mM)
3	1	reservoir	spermine	25 (mM)
4	1	reservoir	MPD	40 (%)