1I2M

RAN-RCC1-SO4 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-06-26
Detector	MARRESEARCH
Wavelength(s)	0.9801
Spacegroup name	P 1
Unit cell lengths	50.334, 71.447, 77.729
Unit cell angles	100.92, 92.05, 104.47

Refinement procedure

Resolution	19.790 - 1.760
Rwork	0.192
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1A12 (RCC1) and human Ran-GDP-Mg2+ structure (Scheffzek K. et al. (1995))
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.790	1.670
High resolution limit [Å]	1.630	1.630
Rmerge	0.146	0.316
Number of reflections	484600
<I/σ(I)>	6.8	0.8
Completeness [%]	92.3	56.1
Redundancy	4.1	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	20 *	20-10% PEG 4000, 200 mM potassium fluoride, 25 mM potassium phosphate, 25 mM ammonium sulfate, 2 mM EDTA, 3mM DTE, pH 6.5, VAPOR DIFFUSION, HANGING DROP
1	VAPOR DIFFUSION, HANGING DROP	6.5	20 *	20-10% PEG 4000, 200 mM potassium fluoride, 25 mM potassium phosphate, 25 mM ammonium sulfate, 2 mM EDTA, 3mM DTE, pH 6.5, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20 (%)
2	1	reservoir	KF	200 (mM)