1I27
CRYSTAL STRUCTURE OF THE C-TERMINAL DOMAIN OF THE RAP74 SUBUNIT OF HUMAN TRANSCRIPTION FACTOR IIF (TFIIF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2000-07-02 |
| Detector | SBC-2 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.052, 43.024, 48.161 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.020 |
| R-factor | 0.126 |
| R-free | 0.14600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.060 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.059 | 0.335 |
| Number of reflections | 58701 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 12.3 | 2.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 4 * | Zinc Sulfate, Lithium chloride, PEG 2000 MME, polyvinylpyrrolidone 15K, glucosamine hydrochloride, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 282K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | MES | 0.1 (M) | |
| 3 | 1 | reservoir | 0.01 (M) | ||
| 4 | 1 | reservoir | 0.1 (M) | ||
| 5 | 1 | reservoir | PEG2000MME | 25 (%(w/v)) | |
| 6 | 1 | reservoir | polyvinylpyrrolidone | 0.5 (%(w/v)) | |
| 7 | 1 | reservoir | glucosamine hydrochloride | 0.1 (M) |
