1I1D
CRYSTAL STRUCTURE OF YEAST GNA1 BOUND TO COA AND GLNAC-6P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.010, 51.619, 91.655 |
| Unit cell angles | 90.00, 107.98, 90.00 |
Refinement procedure
| Resolution | 24.120 - 1.800 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i12 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 * | 0.412 * |
| Number of reflections | 62765 | |
| <I/σ(I)> | 12.5 | 1.8 |
| Completeness [%] | 97.4 | 96.3 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 20 * | PEG 600, IMIDAZOLE/MALATE, pH 5.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG600 | 17-22 (%) | |
| 2 | 1 | reservoir | imidazole | 0.2 (M) | |
| 3 | 1 | reservoir | malate | 0.4 (M) | |
| 4 | 1 | drop | Tris-HCl | 10 (mM) | |
| 5 | 1 | drop | 150 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | protein | 10 (mg/ml) |
