1I1D

CRYSTAL STRUCTURE OF YEAST GNA1 BOUND TO COA AND GLNAC-6P

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-11-27
Detector	MARRESEARCH
Wavelength(s)	1.1000
Spacegroup name	C 1 2 1
Unit cell lengths	156.010, 51.619, 91.655
Unit cell angles	90.00, 107.98, 90.00

Refinement procedure

Resolution	24.120 - 1.800
R-factor	0.183
Rwork	0.183
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i12
RMSD bond length	0.019
RMSD bond angle	1.800
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.048 *	0.412 *
Number of reflections	62765
<I/σ(I)>	12.5	1.8
Completeness [%]	97.4	96.3
Redundancy	2.2	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	20 *	PEG 600, IMIDAZOLE/MALATE, pH 5.10, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG600	17-22 (%)
2	1	reservoir	imidazole	0.2 (M)
3	1	reservoir	malate	0.4 (M)
4	1	drop	Tris-HCl	10 (mM)
5	1	drop		150 (mM)
6	1	drop	dithiothreitol	1 (mM)
7	1	drop	protein	10 (mg/ml)