1I0M
1.05 A STRUCTURE OF THE A-DECAMER GCGTATACGC WITH A SINGLE 2'-O-FLUOROETHYL THYMINE IN PLACE OF T6, HIGH RB-SALT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.820, 45.260, 43.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.050 |
| R-factor | 0.154 |
| Rwork | 0.154 |
| R-free | 0.17000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.050 |
| Rmerge | 0.046 |
| Number of reflections | 22802 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | Rb salt, pH 6.0, VAPOR DIFFUSION, HANGING DROP at 298 K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA | 1 (mM) | |
| 2 | 1 | drop | alkali metal ions(chloride salt) | 40 (mM) | |
| 3 | 1 | drop | alkaline cacodylate | 20 (mM) | |
| 4 | 1 | drop | spermine | 6 (mM) | |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | reservoir | MPD | 35 (%) |
