1I0A

CRYSTAL STRUCTURE OF WILD TYPE TURKEY DELTA 1 CRYSTALLIN (EYE LENS PROTEIN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1993-05-30
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	89.420, 146.550, 152.899
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.780 - 2.500
R-factor	0.156
Rwork	0.156
R-free	0.21300
Structure solution method	MIR
RMSD bond length	0.005
RMSD bond angle	18.900 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	MLPHARE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.500
Rmerge	0.107
Number of reflections	68630
Completeness [%]	97.0
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	293	0.1M calcium acetate, 7-15% PEG 6000, argininosuccinate, pH 4.7, VAPOR DIFFUSION, HANGING DROP at 293 K