1HZY
HIGH RESOLUTION STRUCTURE OF THE ZINC-CONTAINING PHOSPHOTRIESTERASE FROM PSEUDOMONAS DIMINUTA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 1999-12-17 |
Detector | SBC-1 |
Wavelength(s) | 0.71 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 128.400, 90.030, 68.400 |
Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.300 |
Rwork | 0.196 |
R-free | 0.22800 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.012 |
RMSD bond angle | 14.580 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | TNT |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.350 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.096 | 0.173 |
Total number of observations | 1448033 * | |
Number of reflections | 186115 | |
<I/σ(I)> | 25 | |
Completeness [%] | 97.0 | 97 |
Redundancy | 3.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 | 4 * | Benning, M.M., (1995) Biochemistry, 34, 7973. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 10 (%) | |
3 | 1 | reservoir | 5 (mM) | ||
4 | 1 | reservoir | 2-phenylethanol | 1 (%(v/v)) | |
5 | 1 | reservoir | CHES | 50 (mM) |