1HZ4
CRYSTAL STRUCTURE OF TRANSCRIPTION FACTOR MALT DOMAIN III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-08-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.845, 87.845, 110.036 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 1.450 |
| R-factor | 0.189 * |
| Rwork | 0.189 |
| R-free | 0.20700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.053 | 0.248 * |
| Number of reflections | 86222 | |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 98.9 * | 99.2 |
| Redundancy | 7.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 18 * | ammonium sulphate, magnesium sulphate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.2K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5 (mg/ml) | |
| 2 | 1 | reservoir | MES/NaOH | 50 (mM) | pH6.5 |
| 3 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
| 4 | 1 | reservoir | magnesium sulfate | 30 (mM) |
