1HZ4

CRYSTAL STRUCTURE OF TRANSCRIPTION FACTOR MALT DOMAIN III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-08-03
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	87.845, 87.845, 110.036
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.000 - 1.450
R-factor	0.189 *
Rwork	0.189
R-free	0.20700
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.470
High resolution limit [Å]	1.450	1.450
Rmerge	0.053	0.248 *
Number of reflections	86222
<I/σ(I)>	7.8
Completeness [%]	98.9 *	99.2
Redundancy	7.9	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	18 *	ammonium sulphate, magnesium sulphate, MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.2K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	reservoir	MES/NaOH	50 (mM)	pH6.5
3	1	reservoir	ammonium sulfate	1.6 (M)
4	1	reservoir	magnesium sulfate	30 (mM)