1HZ1
RIBONUCLEASE T1 V16A MUTANT IN COMPLEX WITH MG2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.515, 47.289, 50.023 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.1964 * |
| Rwork | 0.196 |
| R-free | 0.21440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.475 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.098 | 0.429 |
| Total number of observations | 81069 * | |
| Number of reflections | 9363 | 926 * |
| <I/σ(I)> | 16.81 | 3.78 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.66 | 6.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | MPD, acetate, calcium chloride, magnesiusm chrloride (soak), pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNase T1 | 15 (mg/ml) | |
| 2 | 1 | drop | exo-cGPS | 15 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | reservoir | MPD | 55 (%(v/v)) |
