1HYN
CRYSTAL STRUCTURE OF THE CYTOPLASMIC DOMAIN OF HUMAN ERYTHROCYTE BAND-3 PROTEIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-D |
| Synchrotron site | APS |
| Beamline | 14-BM-D |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 1998-09-23 |
| Detector | ADSC QUANTUM 1 |
| Wavelength(s) | 0.9789, 0.9796, 0.9537 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.590, 92.580, 123.330 |
| Unit cell angles | 90.00, 131.86, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.216 * |
| Rwork | 0.216 |
| R-free | 0.29000 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 * |
| RMSD bond angle | 1.210 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 * | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.057 * | 0.208 * |
| Number of reflections | 46609 | |
| <I/σ(I)> | 37.16 | 18.7 |
| Completeness [%] | 99.1 * | 94.5 * |
| Redundancy | 3.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.4 * | Kiyatkin, A., (1995) Proteins: Struct., Funct., Genet., 22, 293. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.2-2 (mg/ml) | |
| 10 | 1 | reservoir | sodium azide | 1 (mM) | |
| 2 | 1 | drop | sodium phosphate | 5 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | sodium azide | 1 (mM) | |
| 6 | 1 | drop | dithiothreitol | 1 (mM) | |
| 7 | 1 | drop | glycerol | 30 (%(v/v)) | |
| 8 | 1 | reservoir | ammonium sulfate | 65-68 (%sat) | |
| 9 | 1 | reservoir | sodium citrate | 150 (mM) |
