1HYL
THE 1.8 A STRUCTURE OF COLLAGENASE FROM HYPODERMA LINEATUM
Experimental procedure
| Collection date | 1989-12-04 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 111.700, 111.700, 165.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 3.000 |
| Data reduction software | MADNES |
| Phasing software | X-PLOR (2.1) |
| Refinement software | X-PLOR (2.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.400 | |
| High resolution limit [Å] | 1.700 | 1.700 * |
| Rmerge | 0.039 | |
| Number of reflections | 42152 | |
| Completeness [%] | 74.0 | 51 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.4 * | 291 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | HLC | 20 (mg/ml) | |
| 2 | 1 | 1 | Tris | 50 (mM) | |
| 3 | 1 | 1 | 60 (mM) | ||
| 4 | 1 | 1 | ammonium sulfate | 1.23 (M) |
