1HY0

CRYSTAL STRUCTURE OF WILD TYPE DUCK DELTA 1 CRYSTALLIN (EYE LENS PROTEIN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X8C
Synchrotron site	NSLS
Beamline	X8C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-07-15
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	100.600, 100.600, 168.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 2.200
R-factor	0.207
Rwork	0.190
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	18.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.080	0.290
Total number of observations	147604 *
Number of reflections	48769
<I/σ(I)>	7.4
Completeness [%]	96.4	94.2
Redundancy	3	2.68

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	15% PEG 550 MME, 10mM zinc sulphate, 100mM MES, pH 6.5. VAPOR DIFFUSION, HANGING DROP at 298 K, temperature 298.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	reservoir	mPEG550	15 (%)
3	1	reservoir		10 (mM)
4	1	reservoir	MES	100 (mM)