1HXS
CRYSTAL STRUCTURE OF MAHONEY STRAIN OF POLIOVIRUS AT 2.2A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-09-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 320.500, 355.250, 377.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.268 |
| Rwork | 0.268 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1asj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.325 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DJF |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 300.000 | 2.190 |
| High resolution limit [Å] | 2.200 * | 2.150 |
| Rmerge | 0.091 | 0.563 |
| Total number of observations | 1778215 * | |
| Number of reflections | 1770661 * | |
| <I/σ(I)> | 5.1 | 1.13 |
| Completeness [%] | 77.4 | 77.2 |
| Redundancy | 1.61 | 1.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7 | 4 * | 10mM Pipes, 50-70 mM NaCl, 5 mM MgCl2, 1mM CaCl2, pH 7.0, MICRODIALYSIS, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 12 (mg/ml) | |
| 2 | 1 | 1 | 50-70 (mM) | ||
| 3 | 1 | 1 | PIPES Na | 10 (mM) | pH7.0 |
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 1 | 1 (mM) |
