1HXM
Crystal Structure of a Human Vgamma9/Vdelta2 T Cell Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-06-28 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.048, 151.736, 97.873 |
| Unit cell angles | 90.00, 99.48, 90.00 |
Refinement procedure
| Resolution | 30.770 - 3.120 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.26600 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.106 | 0.700 |
| Number of reflections | 42118 | |
| <I/σ(I)> | 20.2 | 2.7 |
| Completeness [%] | 98.8 | 97.6 |
| Redundancy | 9.8 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | 28% PEG4000, 0.2 M Li2SO4, 0.1 M Tris-HCl, 20% glycerol, 4 mg/mL protein, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 28 (%) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 5 | 1 | drop | glycerol | 20 (%) |
