1HXD

CRYSTAL STRUCTURE OF E. COLI BIOTIN REPRESSOR WITH BOUND BIOTIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	AREA DETECTOR
Collection date	1999-09-02
Detector	UCSD MARK II
Wavelength(s)	1.5418
Spacegroup name	C 2 2 21
Unit cell lengths	104.900, 108.900, 143.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.000 - 2.400
Rwork	0.189
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bia
RMSD bond length	0.018
RMSD bond angle	2.800
Data reduction software	SDMS
Data scaling software	SDMS
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000	2.590
High resolution limit [Å]	2.400	2.400
Rmerge	0.071	0.183
Total number of observations	48934 *
Number of reflections	26762
<I/σ(I)>	9	1.8
Completeness [%]	83.0	33 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	reservoir	sodium potassium phosphate	2.3 (M)