1HWM

EBULIN,ORTHORHOMBIC CRYSTAL FORM MODEL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-05-11
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	57.330, 97.690, 113.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.194
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Ricin(PDB 2AAI) non-conserved residues truncated to alanines
RMSD bond length	0.004
RMSD bond angle	30.771 *
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.093	0.321
Number of reflections	13577
<I/σ(I)>	8.9
Completeness [%]	86.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5 *	4 *	1M Lithium Sulfate, 2% PEG 8000,100 mM Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		1.0 (M)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	drop	protein	10-15 (mg/ml)